中英
1
labeling technique中文是什么意思
- 中文翻譯
- 造句
- 標(biāo)記原子
- label n. 1.紙條，貼條，標(biāo)簽，簽條。 2.稱號，綽號。 3 ...
- technique n. 1.(專門)技術(shù)；(藝術(shù)上的)技巧，技能。 2.手 ...
- affinity labeling technique 親和標(biāo)記技術(shù),親和示蹤技術(shù)
- cell labeling technique 細胞標(biāo)記技術(shù)
- deuterium labeling technique 氘標(biāo)記術(shù)
- fluorigenic labeling technique 熒光生成標(biāo)記技術(shù); 致熒光標(biāo)記技術(shù)
- pulse-labeling technique 脈沖標(biāo)記技術(shù)
- tdt mediated nick-end labeling technique tdt介導(dǎo)槽口端標(biāo)志法
- labeling 標(biāo)號; 標(biāo)簽費 標(biāo)貼 標(biāo)志; 車嘜; 導(dǎo)入示蹤原子; 定名; 加標(biāo)記; 加標(biāo)簽，貼標(biāo)簽; 貼商標(biāo); 貼紙; 張貼危險告示；作標(biāo)記; 作標(biāo)記的
- afferent labeling 傳入標(biāo)記
- affinity labeling 親和標(biāo)記; 親合標(biāo)記法
- antibody labeling 抗體標(biāo)記
- asymmetric labeling 不對稱標(biāo)記
- biotin labeling 生物素標(biāo)記
- certification and labeling 認(rèn)證與商標(biāo)
- chemiluminescent labeling 化學(xué)發(fā)光標(biāo)記（法）
- cohort labeling 同類標(biāo)記; 同群標(biāo)記
- cold labeling 冷標(biāo)記
- conjugation labeling 聯(lián)接標(biāo)記
- cosmetics labeling 化妝品標(biāo)簽
- custom labeling 委托標(biāo)記
- cyclic labeling 循環(huán)標(biāo)定
- descriptive labeling 品種簡介標(biāo)簽; 商品簡介標(biāo)簽
- deuterium labeling 氘標(biāo)記; 氘標(biāo)志；氘標(biāo)識法
- diagram labeling 圖解標(biāo)簽題
- By using the gfp - labeling technique , we have found that gfp - cam colocalized with all the characterized structures formed during cytokinesis
通過采用gfp標(biāo)記技術(shù)，我們觀察到gfp - cam與胞質(zhì)分裂期細胞內(nèi)形成的特征結(jié)構(gòu)存在共分布。 - But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area " . to answer these questions , we observed systemically the synaptic connections among the pv - like immunoreactive neurons , fibers and terminals and the connections between dnpi - like , gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry , triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique
但是目前對新發(fā)現(xiàn)的囊泡膜mu轉(zhuǎn)運體一dnn樣陽性終末與帶狀區(qū)內(nèi)pag樣陽性神經(jīng)元之間ej關(guān)系，以及谷氨酸脫發(fā)酶（ gad ，是gaba能神經(jīng)元和終末的特異性標(biāo)識物）是否參與其調(diào)控作用，尚缺乏系統(tǒng)的形態(tài)學(xué)資料。 - The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation . 2 . following lps or seb was administered intraperitoneally , the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice . double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔內(nèi)給予細菌內(nèi)毒素lps或腸毒素seb ，用免疫組織化學(xué)方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達，并采用雙標(biāo)記技術(shù)觀察了1型il刁受體陽性神經(jīng)元和加壓素及催產(chǎn)素表達的關(guān)系。 - The amplification system was optimized so that the pcr with different primers can be carried out under the same condition . the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques . sequences were analyzed and compared base on sequencing analysis3 . 4 and seq / ede software
方法根據(jù)mtdna控制區(qū)及其周圍區(qū)域的序列，設(shè)計多對引物，探索優(yōu)化擴增體系，使擴增條件能夠同時滿足多對引物的需要，用sanger末端終止法及熒光標(biāo)記技術(shù)對樣本進行dna測序， sequencinganalysis3 . 4和seq ede軟件進行序列分析和比對。 - The characteristics of this method are : a , directly counting cell number without the influence of the metabolic state of the cells ; b , discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c , less treatment step , and free - radioactivity ; d , high sensitivity and reliability . 2 , using the above assay , immunofluorescent labeled technique , and flow cytometry , the pbmc proliferation , apoptosis , necrosis , cell cycle , activation , cytokines and membrane marker were detected . the results showed that the number of pbmc reduced , but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle , but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結(jié)合以上創(chuàng)建的方法和免疫熒光流式細胞術(shù)，用k562細胞株可溶性分泌物(上清)對外周血單個核細胞( pbmc )進行培養(yǎng)以模擬體內(nèi)微環(huán)境，然后分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結(jié)果發(fā)現(xiàn)用腫瘤上清培養(yǎng)的pbmc細胞數(shù)量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數(shù)的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，并促進表達th1 、 th2細胞因子。 - The self - segregation behavior of amphiphilic copolymer on pdl - la scaffold was investigated via fluorescence - labeling technique . the modified scaffold with hydrophilic surface will not only favor the penetration of cell suspension and culture medium , but also provide the microenvironment for the growth of cells with the peo spacer combining amino acid ( rgd ) structure . according the above result , the cytocompatibility test was also performed on pdl - la 3d scaffold modified by amphiphilic copolymer with alkaline amino acid end
這種親水表面不僅有利于細胞懸液和培養(yǎng)介質(zhì)的進入，并可以通過peo橋聯(lián)的氨基酸( rgd )為細胞在三維多孔支架內(nèi)的生長提供類細胞外基質(zhì)環(huán)境；根據(jù)以上結(jié)果，本文對堿性氨基酸為peo鏈端基的兩親共聚物-氨基酸類細胞外基質(zhì)修飾的聚乳酸三維支架進行了細胞相容性的測試。 - However , the advance of intracellular labeling techniques enables us not only to visualize more complete dendritic arbor for qualitative analysis , but also to examine the relation between changes in the dendritic arborization and the evoked fast postsynaptic curents - 3 - ( fpscs ) in the same neurons during the postnatal development the aim of this study was to systematically examine the postnatal changes in the configuration of fpscs evoked by the focal stimulation of the stratum radiatum of the ca1 region , and the relationship between the dendritic arborization and evoked fpscs in the rat hippocampal ca1 pyramidal neurons using whole - cell blind patch recording technique combined with biocytin intracellular labeling during the postnatal development ( postnatal day 2 - 70 , p2 - p70 )
但是，細胞內(nèi)染色技術(shù)的進步使我們不僅能觀察到更完整的樹突分支來用于定性研究，而且也可以在同一神經(jīng)元上研究在發(fā)育過程中樹突分支的變化與誘發(fā)的快突觸后電流( fastpostsynapticcurrents , fpscs )之間的關(guān)系。因此，本研究應(yīng)用盲法腦片膜片鉗記錄并結(jié)合biocytin細胞內(nèi)染色方法，對發(fā)育過程中(生后2 70天)局部刺激大鼠海馬ca1區(qū)輻射層在錐體神經(jīng)元誘發(fā)的fpscs的成分變化，以及ca1錐體神經(jīng)元的樹突分支與誘發(fā)的fpscs的關(guān)系進行了較為系統(tǒng)的研究。
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